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Registrering og fjernelse af nukleasen forurening under oprensning af rekombinante Prototype skummende Virus Integrase
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Registrering og fjernelse af nukleasen forurening under oprensning af rekombinante Prototype skummende Virus Integrase

DOI:
10.3791/56605-v

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07:50 min

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December 08, 2017

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, , ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Capítulos

  • 00:05Título
  • 00:35Nickel Affinity Chromatography
  • 03:30Heparin Affinity Chromatography
  • 04:28Nuclease Assay
  • 06:46Results: Nuclease Assay of Heparin Sepharose Fractions
  • 07:19Conclusion

Summary

Tadução automática

Tadução automática

Rekombinant prototype skummende virus integrase protein er ofte forurenet med en bakteriel nukleasen under rensningen. Denne metode identificerer nukleasen forurening og fjerner det fra den endelige udarbejdelse af enzymet.

Tags

Nuclease ContaminationProtein PurificationRecombinant Prototype Foamy Virus IntegraseE. ColiSonicationUltracentrifugationFPLCNickel-charged ResinSDS-PAGEUV Absorbance

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