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シリアル セクション電子線トモグラフィーによるショウジョウバエ間接飛翔筋のミトコンドリア微細構造 3D
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
3D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography
DOI:

06:45 min

December 19, 2017

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Capítulos

  • 00:05Título
  • 00:37Sectioning Drosophila Tissues with Vibrating Blade Microtome
  • 01:19High-pressure Freezing (HPF) and Freeze Substitution (FS) for Electron Microscope (EM)
  • 02:30Serial-sectioning for Electron Tomography
  • 03:42Double-title Electron Tomography
  • 05:09Results: Integrated Intra-mitochondria Cristae and Matrix Patterns in 3D
  • 06:20Conclusion

Summary

Tadução automática

このプロトコルではシリアル セクション電子線トモグラフィーのショウジョウバエ間接飛翔筋のミトコンドリアの構造を解明への応用を示す.

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