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连续切片电子断层扫描显示的 3D果蝇间接飞行肌的线粒体超微结构研究
JoVE Journal
Biologia
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JoVE Journal Biologia
3D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography
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连续切片电子断层扫描显示的 3D果蝇间接飞行肌的线粒体超微结构研究

DOI:
10.3791/56567-v

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06:45 min

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December 19, 2017

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, ,
1Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine,National Taiwan University, 2Institute of Cellular and Organismic Biology,Academia Sinica

Capítulos

  • 00:05Título
  • 00:37Sectioning Drosophila Tissues with Vibrating Blade Microtome
  • 01:19High-pressure Freezing (HPF) and Freeze Substitution (FS) for Electron Microscope (EM)
  • 02:30Serial-sectioning for Electron Tomography
  • 03:42Double-title Electron Tomography
  • 05:09Results: Integrated Intra-mitochondria Cristae and Matrix Patterns in 3D
  • 06:20Conclusion

Summary

Tadução automática

Tadução automática

在本协议中, 我们展示了在果蝇间接飞行肌肉中应用的序列切片电子断层扫描来阐明线粒体结构。

Tags

Keywords: 3DMitochondrial UltrastructureDrosophila Indirect Flight MuscleSerial-section Electron TomographyCellular UltrastructureHigh-pressure FreezingFree SubstitutionResin EmbeddingUltramicrotomyTransmission Electron MicroscopeDual-axis Tomography

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