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Stereotactically-guided Ablation of the Rat Auditory Cortex, and Localization of the Lesion in the Brain
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JoVE Journal Comportamento
Stereotactically-guided Ablation of the Rat Auditory Cortex, and Localization of the Lesion in the Brain

Stereotactically-guided Ablation of the Rat Auditory Cortex, and Localization of the Lesion in the Brain

DOI:
10.3791/56429-v

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09:29 min

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October 11, 2017

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, , , ,
1Institute of Neuroscience of Castilla y León,University of Salamanca, 2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Department of Otolaryngology,Harvard Medical School

Capítulos

  • 00:05Título
  • 01:16Location of the AC in the Temporal Bone of the Rat
  • 03:04Surgical Exposure and Ablation of the AC
  • 05:07Tissue Collection
  • 06:18Localization of the AC Lesions
  • 07:56Results: Changes in mRNA Levels of AMPA Receptor Subunits GluA2 and GluA3 After Unilateral AC Ablation 7 Days Post-Lesion
  • 08:38Conclusion

Summary

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We describe a method for the stereotactically-guided location, exposure, and ablation of the auditory cortex in rats. The localization of the ablation is assessed using a coordinate map postmortem.

Tags

Stereotactic AblationAuditory CortexRat BrainAuditory NeuroscienceCortical PlasticityUnilateral LesionSurgical TechniqueCoordinate MappingBregmaLambdaInteraural LineTemporal Bone ExposureCraniotomy

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