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活细胞 Sod1 突变蛋白聚集物高含量定量测定方法的建立

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

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活细胞 Sod1 突变蛋白聚集物高含量定量测定方法的建立

DOI:

10.3791/56425-v

•

11:12 min

•

October 04, 2017

•

Honggun Lee, Constantin Radu, Jeung Whan Han, Regis Grailhe

¹Automation & Logistics Management, Screening Sciences & Novel Assay Technologies,Institut Pasteur Korea, ²School of Pharmacy,Sungkyunkwan University, ³Technology Development Platform,Institut Pasteur Korea

Capítulos

00:05Título
00:58Lentivirus Production
02:50Lentiviral Transduction and Stable Line Generation
04:53Dose Response Effect of Proteosome Inhibitors on Protein Aggregation and Image Analysis
07:06Time lapse Imaging of YFP Expression in Living Cells
08:09Results: Quantification of SOD1 Mutant Protein Aggregate Formation
10:04Conclusion

Summary

Tadução automática

我们描述一种方法来量化错误蛋白质的聚集。我们的协议细节慢诱导稳定细胞线生成, 自动共焦成像, 和图像分析的蛋白质集料。作为一个说明性应用, 我们研究了小分子在促进 SOD1 聚集的时间和剂量依赖性的方式的影响。

Tags

Keywords: Assay Development High Content Quantification Sod1 Mutant Protein Protein Aggregate Formation Living Cells Neurodegenerative Disorders ALS Lentivirus Production HEK-293 T-cells Transfection Cell Transduction

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