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Live bilder av GLUT4 Protein menneskehandel musen primære hypothalamus nevroner bruker Deconvolution mikroskopi
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1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Capítulos
- 00:05Título
- 01:00Primary Neuron Culture and Brain Tissue Isolation
- 02:56Tissue Digestion, Plating, and Culture
- 04:32Live Image Recording
- 06:48Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
- 07:47Conclusion
Denne protokollen beskriver en teknikk for observasjon av sanntids Green fluorescens Protein (GFP) merket glukose Transporter 4 (GLUT4) protein handel på insulin stimulering og karakterisering av biologiske rollen CCR5 i insulin-GLUT4 signalveien med Deconvolution mikroskopi.
