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Sanntid i Vivo innspilling av Arabidopsis kalsium signaler under insekt fôring med et fluorescerende Biosensor
1Department of Metabolic Biology,John Innes Centre, Norwich Research Park, 2Department of Botany,University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology,Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO),Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology,John Innes Centre, Norwich Research Park
Capítulos
- 00:05Título
- 00:44Arabidopsis Leaf Detachment
- 01:38Fluorescence Microscopy
- 03:06Data Collection
- 05:39Time-course Video Creation
- 06:39Results: Analysis of GCaMP3 Fluorescence to Detect Aphid-elicited Calcium Signals at the Feeding Site in Detached Arabidopsis Leaves
- 07:30Conclusion
Denne protokollen skisserer en enkel metode for å analysere kalsium signaler i planter generert av fôring hemipteran insekter, som bladlus. Arabidopsis thaliana forvandles med GFP kalsium biosensor GCaMP3 gir sanntid i vivo avbilding av kalsium dynamics med høy timelige og romlig oppløsning.
