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Beoordeling van Dopaminerge homeostase in muizen door gebruik van High-performance Liquid Chromatography analyse en Synaptosomal Dopamine opname
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1Molecular Neuropharmacology and Genetics Laboratory, Lundbeck Foundation Center for Biomembranes in Nanomedicine, Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences,University of Copenhagen, 2Laboratory of Neuropsychiatry, Psychiatric Center Copenhagen and Department of Neuroscience and Pharmacology,University of Copenhagen
Capítulos
- 00:05Título
- 01:00Synaptosomal Preparation
- 03:22Uptake Experiment
- 07:00High-performance Liquid Chromatography Analysis
- 09:19Results: Representative Results from a Synaptosomal DA Uptake Experiment of Four C57BI/6 Wild Type Mice from Drd1a-cre Mouse Strain
- 10:40Conclusion
Synaptosomal dopamine opname en krachtige vloeibare chromatografie vertegenwoordigen experimentele analysetools te onderzoeken van dopamine homeostase in muizen door beoordeling van de functie van de dopamine transporter en niveaus van dopamine in striatale weefsel, respectievelijk. Hier presenteren we protocollen voor dopamine weefsel inhoud meten en beoordelen van de functionaliteit van de dopamine transporter.
