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高效液相色谱分析和触多巴胺摄取对小鼠多巴胺能稳态的评价

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake

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高效液相色谱分析和触多巴胺摄取对小鼠多巴胺能稳态的评价

DOI:

10.3791/56093-v

•

11:26 min

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September 21, 2017

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Kathrine L. Jensen, Annika H. Runegaard, Pia Weikop, Ulrik Gether, Mattias Rickhag

¹Molecular Neuropharmacology and Genetics Laboratory, Lundbeck Foundation Center for Biomembranes in Nanomedicine, Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences,University of Copenhagen, ²Laboratory of Neuropsychiatry, Psychiatric Center Copenhagen and Department of Neuroscience and Pharmacology,University of Copenhagen

Capítulos

00:05Título
01:00Synaptosomal Preparation
03:22Uptake Experiment
07:00High-performance Liquid Chromatography Analysis
09:19Results: Representative Results from a Synaptosomal DA Uptake Experiment of Four C57BI/6 Wild Type Mice from Drd1a-cre Mouse Strain
10:40Conclusion

Summary

Tadução automática

触多巴胺摄取和高效液相色谱分析是通过评估多巴胺转运体的功能和纹组织中多巴胺的水平来研究小鼠体内多巴胺稳态的实验工具,分别.在这里, 我们提出了测量多巴胺组织含量和评估多巴胺转运体功能的协议。

Tags

Keywords: Dopamine Dopamine Transporter HPLC Synaptosomal Dopamine Uptake Dopamine Homeostasis In Vivo Manipulation Brain Dissection Synaptosome Preparation Dopamine Uptake Assay

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