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יישום אוטומטי של תמונה מודרך תיקון מהדק לחקר נוירונים של פרוסות המוח
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Neurociência
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JoVE Journal Neurociência
Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
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יישום אוטומטי של תמונה מודרך תיקון מהדק לחקר נוירונים של פרוסות המוח

11,175 Views

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09:05 min

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July 31, 2017

DOI:

10.3791/56010-v

DOI:
10.3791/56010-v

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09:05 min
July 31, 2017

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2 Views
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1Department of Biological Sciences,Purdue University, 2Purdue Institute for Integrative Neuroscience,Purdue University

Tags

Automated Image-guided Patch ClampNeuronsBrain SlicesComputer VisionFluorescent CellsPatch Clamp ElectrophysiologyHigh ThroughputNeurological DisordersPharmacological ScreeningNeuronal PreparationsBrain SliceSlice CultureDissociated NeuronsCalibrationManipulatorMicroscopeAmplifierAutopatcher IGInternal SolutionPipette TipMicroscope StageCoordinate System

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Cite this Article

Wu, Q., Chubykin, A. A. Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices. J. Vis. Exp. (125), e56010, doi:10.3791/56010 (2017).

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