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Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
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Neurociência
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JoVE Journal Neurociência
Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

DOI:
10.3791/56010-v

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09:05 min

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July 31, 2017

•

,
1Department of Biological Sciences,Purdue University, 2Purdue Institute for Integrative Neuroscience,Purdue University

Capítulos

  • 00:05Título
  • 01:16Run Autopatcher IG
  • 02:48Automatic Patch Clamp Procedure
  • 07:03Results: Conducting an Automated Drug Delivery Experiment
  • 08:09Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes how to conduct automatic image-guided patch-clamp experiments using a system recently developed for standard in vitro electrophysiology equipment.

Tags

Keywords: Automated Image-guided Patch ClampNeuronsBrain SlicesComputer VisionFluorescent CellsPatch Clamp ElectrophysiologyHigh ThroughputNeurological DisordersPharmacological ScreeningNeuronal PreparationsBrain SliceSlice CultureDissociated NeuronsCalibrationManipulatorMicroscopeAmplifierAutopatcher IGInternal SolutionPipette TipMicroscope StageCoordinate System

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