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Locust AntennaeにおけるRNAによる嗅覚受容体遺伝子の局在<em>現場で</em>ハイブリダイゼーション
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Neurociência
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JoVE Journal Neurociência
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
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Locust AntennaeにおけるRNAによる嗅覚受容体遺伝子の局在<em>現場で</em>ハイブリダイゼーション

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09:30 min

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July 13, 2017

DOI:

10.3791/55924-v

DOI:
10.3791/55924-v

•

09:30 min
July 13, 2017

•

2 Views
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1Department of Entomology,China Agricultural University, 2Bio-tech Research Center,Shandong Academy of Agricultural Sciences

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Odorant Receptor GenesRNA In Situ HybridizationLocust AntennaeDigoxigenin Labeled ProbesBiotin Labeled ProbesFreezing MicrotomeCryostat Sections4% PFA0.2 M HCl1X PBS1% Triton X-100FormamideAntisense ProbeSense Probe0.1X SSC1X TBS1% Blocking ReagentChromogenic Detection

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Cite this Article

Xu, X., You, Y., Zhang, L. Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization. J. Vis. Exp. (125), e55924, doi:10.3791/55924 (2017).

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