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通过RNA定位蝗虫天线中的气味受体基因<em>原位</em>杂交

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

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通过RNA定位蝗虫天线中的气味受体基因<em>原位</em>杂交

7,952 Views

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09:30 min

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July 13, 2017

DOI:

10.3791/55924-v

DOI:

10.3791/55924-v

•

09:30 min

July 13, 2017

•

2 Views

Xiao Xu, Yinwei You², Long Zhang

¹Department of Entomology,China Agricultural University, ²Bio-tech Research Center,Shandong Academy of Agricultural Sciences

Tags

Odorant Receptor Genes RNA In Situ Hybridization Locust Antennae Digoxigenin Labeled Probes Biotin Labeled Probes Freezing Microtome Cryostat Sections 4% PFA 0.2 M HCl 1X PBS 1% Triton X-100 Formamide Antisense Probe Sense Probe 0.1X SSC 1X TBS 1% Blocking Reagent Chromogenic Detection

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