Journal
/
Neurociência
/
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

DOI:
10.3791/55924-v

•

09:30 min

•

July 13, 2017

•

, ,
1Department of Entomology,China Agricultural University, 2Bio-tech Research Center,Shandong Academy of Agricultural Sciences

Capítulos

  • 00:05Título
  • 00:55Preparation of Cryostat Sections
  • 01:59Fixing Sections and Hybridization
  • 03:30Washing, Blocking, and Immunohistochemistry
  • 05:10Staining
  • 07:02Results: Neuronal Identity of Antennal Cells Expressing LmigORs by Chromogenic and TSA Detections
  • 08:27Conclusion

Summary

Tadução automática

Tadução automática

This paper describes a detailed and highly effective RNA in situ hybridization protocol particularly for low-level expressed Odorant Receptor (OR) genes, as well as other genes, in insect antennae using digoxigenin (DIG)-labeled or biotin-labeled probes.

Tags

Odorant Receptor GenesRNA In Situ HybridizationLocust AntennaeDigoxigenin Labeled ProbesBiotin Labeled ProbesFreezing MicrotomeCryostat Sections4% PFA0.2 M HCl1X PBS1% Triton X-100FormamideAntisense ProbeSense Probe0.1X SSC1X TBS1% Blocking ReagentChromogenic Detection

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Double Fluorescence in situ Hybridization in Fresh Brain Sections

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Read Article