/

/

与ChEC-seq的蛋白质-DNA相互作用的全基因组映射<em>酿酒酵母（Saccharomyces cerevisiae）</em

JoVE Journal
Genética

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Genética

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Please note that all translations are automatically generated. Click here for the English version.

与ChEC-seq的蛋白质-DNA相互作用的全基因组映射<em>酿酒酵母（Saccharomyces cerevisiae）</em

DOI:

10.3791/55836-v

•

10:43 min

•

June 03, 2017

•

Sebastian Grünberg, Gabriel E. Zentner

¹Basic Sciences Division,Fred Hutchinson Cancer Research Center, ²Department of Biology,Indiana University

Capítulos

00:05Título
00:49Chromatin Endogenous Cleavage (ChEC)
06:30Size Selection
08:28Results: ChEC-seq Enables Genome-wide Mapping of Protein-DNA Interactions
09:54Conclusion

Summary

Tadução automática

我们描述了染色质内源性切割与高通量测序（ChEC-seq），染色质免疫沉淀（ChIP） - 正交法，用于在全基因组范围内用微球菌核酸酶（MNase）融合蛋白测定蛋白质结合位点。

Tags

Keywords: ChEC-seq Protein-DNA Interactions Genome-wide Mapping Saccharomyces Cerevisiae Transcription Chromatin DNA Binding Regulatory Factors High Resolution Low Background Formaldehyde Crosslinking Chromatin Solubilization Antibodies MNase Calcium Addition Stop Solution SDS

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

通过DNA含量测定的哺乳动物复制时序的全基因组的测定

使用生物素化补骨脂素全球定位RNA-RNA相互作用

ATAC 基因组在原发性人类 T 淋巴细胞中的染色体全易接近染色质

增殖和静止人成纤维细胞的全基因组转录衰变率的测定

大范围早期果蝇胚胎的全基因组染色质构象捕获库的生成

从酿酒酵母中组蛋白修饰的染色质沉淀 (芯片)

启动子捕获高分辨率, 全基因组的启动子交互分析

全基因组对真核生物转录误差的监测

酿酒酵母4-硫尿嘧啶代谢标记及新合成 mRNA 作为 RNA 聚合酶 II. 活性的一种代理

通过全基因组互惠性分析,对糖母菌菌种间热耐性差异的遗传图谱

Read Article