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לחיות תאים מדידה של Odorant קולטן הפעלה באמצעות Assay המחנה בזמן אמת

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

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לחיות תאים מדידה של Odorant קולטן הפעלה באמצעות Assay המחנה בזמן אמת

8,669 Views

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09:11 min

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October 02, 2017

DOI:

10.3791/55831-v

DOI:

10.3791/55831-v

•

09:11 min

October 02, 2017

•

8 Views

Yuetian Zhang*¹, Yi Pan*¹, Hiroaki Matsunami³, Hanyi Zhuang⁴

¹Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education,Shanghai Jiao Tong University School of Medicine, ²Department of Molecular Genetics and Microbiology,Duke University Medical Center, ³Department of Neurobiology, Duke Institute for Brain Sciences,Duke University Medical Center, ⁴Institute of Health Science,Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

Tags

Odorant Receptor CAMP Assay Live-cell Measurement High-throughput Screening Heterologous Expression System Hana3A Cells Transfection Odorant-to-receptor Activation Phase-contrast Microscopy Cell Culture Trypsinization Cell Counting 96-well Plate Incubation Plasmid Constructs Transfection Mixture

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