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Live-celle måling af lugtstof Receptor aktivering ved hjælp af en Real-time lejr Assay
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Neurociência
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JoVE Journal Neurociência
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Live-celle måling af lugtstof Receptor aktivering ved hjælp af en Real-time lejr Assay

DOI:
10.3791/55831-v

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09:11 min

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October 02, 2017

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, , ,
1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education,Shanghai Jiao Tong University School of Medicine, 2Department of Molecular Genetics and Microbiology,Duke University Medical Center, 3Department of Neurobiology, Duke Institute for Brain Sciences,Duke University Medical Center, 4Institute of Health Science,Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

Capítulos

  • 00:05Título
  • 00:50Plating and Transfection of Hana3A Cells
  • 03:03Transfection of Plasmids
  • 05:24Stimulation and Measuring OR Activity Using the Real-time cAMP Assay
  • 07:27Results: Kinetic Measurements of the Response of OR5AN1 to Muscone
  • 08:23Conclusion

Summary

Tadução automática

Tadução automática

Kendetegner funktionen af lugtstof receptorer serverer en uundværlig del i deorphanization-processen. Vi beskriver en metode til at måle aktivering af lugtstof receptorer i realtid ved hjælp af en lejr assay.

Tags

Keywords: Odorant ReceptorCAMP AssayLive-cell MeasurementHigh-throughput ScreeningHeterologous Expression SystemHana3A CellsTransfectionOdorant-to-receptor ActivationPhase-contrast MicroscopyCell CultureTrypsinizationCell Counting96-well PlateIncubationPlasmid ConstructsTransfection Mixture

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