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从人体二尖瓣提取蛋白质的优化方案
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Bioquímica
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JoVE Journal Bioquímica
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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从人体二尖瓣提取蛋白质的优化方案

DOI:
10.3791/55762-v

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09:13 min

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June 14, 2017

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, , , , , ,
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan,Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section,University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit,Centro Cardiologico Monzino IRCCS

Capítulos

  • 00:05Título
  • 01:17Mitral Valve Preparation
  • 03:39Protein Extraction
  • 07:20Results: Four Newly Identified Mitral Valve Proteins
  • 08:28Conclusion

Summary

Tadução automática

Tadução automática

人二尖瓣的蛋白质组成仍然是部分未知的，因为其分析由于细胞数量低，因此通过低蛋白质生物合成而复杂化。这项工作提供了有效提取蛋白质用于二尖瓣蛋白质组分析的方案。

Tags

Protein ExtractionMitral ValveCardiac Valve DiseaseProteomic AnalysisDiagnostic MarkersTherapeutic TargetsPorcine Mitral ValveExplanted HeartLeft AtriumLeft VentricleAnterior Mitral Valve LeafletPosterior Mitral Valve LeafletCommissuresDissectionProtein Extraction Workflow

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