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Analysera superkomplex av den mitokondriella elektrontransportkedjan med naturliga elektrofores, in-gel-analyser och elektrolys
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Bioquímica
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JoVE Journal Bioquímica
Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
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Analysera superkomplex av den mitokondriella elektrontransportkedjan med naturliga elektrofores, in-gel-analyser och elektrolys

13,749 Views

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08:37 min

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June 01, 2017

DOI:

10.3791/55738-v

DOI:
10.3791/55738-v

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08:37 min
June 01, 2017

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6 Views
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1Department of Pediatrics-Division Cardiology,University of Rochester

Tags

Mitochondrial Electron Transport ChainSupercomplexesNative ElectrophoresisIn-gel AssaysElectroelutionComplex-1 AssayComplex-5 AssayDetergentsNADHOligomycinNitro Blue TetrazoliumLead NitrateATPBioenergeticsPhysiological IntegrityMembrane Protein Complexes

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Beutner, G., Porter Jr., G. A. Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution. J. Vis. Exp. (124), e55738, doi:10.3791/55738 (2017).

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