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Protokol for differentieringen af ​​humane inducerede pluripotente stamceller i blandede kulturer af neuroner og glia til neurotoksicitetstestning
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Protokol for differentieringen af ​​humane inducerede pluripotente stamceller i blandede kulturer af neuroner og glia til neurotoksicitetstestning

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09:02 min

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June 09, 2017

DOI:

10.3791/55702-v

DOI:
10.3791/55702-v

•

09:02 min
June 09, 2017

•

14 Views
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1Directorate F – Health, Consumers and Reference Materials,Joint Research Centre

Tags

Human Induced Pluripotent Stem CellsNeural Stem CellsNeuronal DifferentiationGlial DifferentiationMixed CulturesNeurotoxicity TestingIn Vitro ModelDrug And Chemical Toxicity ScreeningSignaling PathwaysHiPSC MediumEB MediumColony FragmentsMatrix-coated Dishes

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Pistollato, F., Canovas-Jorda, D., Zagoura, D., Price, A. Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing. J. Vis. Exp. (124), e55702, doi:10.3791/55702 (2017).

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