Journal
/
Biologia do Desenvolvimento
/
将人诱导的多能干细胞分化为神经元和神经胶质的混合培养物进行神经毒性测试的方案
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
Please note that all translations are automatically generated. Click here for the English version.

将人诱导的多能干细胞分化为神经元和神经胶质的混合培养物进行神经毒性测试的方案

DOI:
10.3791/55702-v

•

09:02 min

•

June 09, 2017

•

, , ,
1Directorate F – Health, Consumers and Reference Materials,Joint Research Centre

Capítulos

  • 00:05Título
  • 01:10Human Induced Pluripotent Stem Cell (hiPSC) Expansion
  • 02:20HiPSC Differentiation into Mixed Neurons and Glia: Generation of Embryoid Bodies
  • 03:41Generation of Neuropithelial Aggragates
  • 04:53Aggregate Dissociation and Neuronal Differentiation
  • 06:36Results: Differentiation of IMR90-hiPSCs into Mixed Cultures of Neurons and Glia
  • 07:53Conclusion

Summary

Tadução automática

Tadução automática

人类诱导的多能干细胞（hiPSC）被认为是用于药物和化学筛选以及开发用于毒性测试的新体外模型（包括神经毒性）的有力工具。这里描述了将hiPSC分化为神经元和神经胶质的详细方案。

Tags

Human Induced Pluripotent Stem CellsNeural Stem CellsNeuronal DifferentiationGlial DifferentiationMixed CulturesNeurotoxicity TestingIn Vitro ModelDrug And Chemical Toxicity ScreeningSignaling PathwaysHiPSC MediumEB MediumColony FragmentsMatrix-coated Dishes

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生

从人多能干细胞的黑素细胞的无饲养推导

从人多能干细胞使用高度可重复的小分子为基础的分化协议心肌细胞规模化生产

诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动

在人多能的体外干细胞向滋养层细胞

人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化

3D Cocultures 星形胶质细胞与人多潜能干细胞神经元的突触电路建模

基于 rna 的人原代成纤维细胞重组成诱导多能干细胞

人类多能干细胞有效分化为肝细胞

人诱导多能干细胞体外生成体细胞衍生物的研究

Read Article