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Biologia do Desenvolvimento
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Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

DOI:
10.3791/55702-v

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09:02 min

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June 09, 2017

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, , ,
1Directorate F – Health, Consumers and Reference Materials,Joint Research Centre

Capítulos

  • 00:05Título
  • 01:10Human Induced Pluripotent Stem Cell (hiPSC) Expansion
  • 02:20HiPSC Differentiation into Mixed Neurons and Glia: Generation of Embryoid Bodies
  • 03:41Generation of Neuropithelial Aggragates
  • 04:53Aggregate Dissociation and Neuronal Differentiation
  • 06:36Results: Differentiation of IMR90-hiPSCs into Mixed Cultures of Neurons and Glia
  • 07:53Conclusion

Summary

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Human induced pluripotent stem cells (hiPSCs) are considered a powerful tool for drug and chemical screening and for the development of new in vitro models for toxicity testing, including neurotoxicity. Here, a detailed protocol for the differentiation of hiPSCs into neurons and glia is described.

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Human Induced Pluripotent Stem CellsNeural Stem CellsNeuronal DifferentiationGlial DifferentiationMixed CulturesNeurotoxicity TestingIn Vitro ModelDrug And Chemical Toxicity ScreeningSignaling PathwaysHiPSC MediumEB MediumColony FragmentsMatrix-coated Dishes

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