Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Protein-tRNA Agarose Gel Retardation Assays for…
JoVE Journal
Biochemistry

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biochemistry
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
 

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function

Article DOI: 10.3791/55638-v • 08:03 min • June 21st, 2017
June 21st, 2017 •
1,2,3, 1, 1, 1, 1
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC), 2Department of Immunology, Complutense University School of Medicine, 3Abvance Biotech srl

Capítulos

Resumo
Automatic Translation

A protocol is presented for the production of tRNA(UUU) and the analysis of tRNA(UUU) in complex with the enzyme TcdA by agarose gel retardation assays.

Tags

Protein-tRNA Agarose Gel Retardation Assays N6-threonylcarbamoyladenosine TcdA Function RNA-binding Proteins Interaction Transfer RNA Key Questions RNA Biology Protein-RNA Interactions Small Amounts Of Protein And RNA Samples Informative Results Protein-tRNA Complexes Ribosomal RNA Messenger RNA RNA Molecule E Coli BL21 DE3 Transformed Expression Vector PET-23D EC Triple-U Low Salt Luria Broth LB Plate Ampicillin Incubator
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter