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Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
JoVE Journal
Biologia
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JoVE Journal Biologia
Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

DOI:
10.3791/55471-v

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07:38 min

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April 09, 2017

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, ,
1MRC Laboratory for Molecular Cell Biology,University College London, 2Centre for Mathematics and Physics in Life Sciences and Experimental Biology (CoMPLEX),University College London, 3Department of Cell and Developmental Biology,University College London

Capítulos

  • 00:05Título
  • 00:50Image Setup and Particle Extraction
  • 02:53Seed Generation and Selection
  • 04:32Model Generation from Seeds
  • 06:11Results: Modeling of Vaccinia with VirusMapper
  • 06:49Conclusion

Summary

Tadução automática

Tadução automática

This manuscript uses the Fiji-based open-source software package VirusMapper to apply single-particle analysis to super-resolution microscopy images in order to generate precise models of nanoscale structure.

Tags

Keywords: Single-particle AnalysisSuper-resolution MicroscopyVirusMapperVirus StructureMacromolecular ComplexesFluorescence MicroscopyImage ProcessingParticle ExtractionROI SelectionSeed Generation

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