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L'identification d'un épitope linéaire des cellules B monoclonales d'anticorps reconnus par le peptide à balayage assistée
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L'identification d'un épitope linéaire des cellules B monoclonales d'anticorps reconnus par le peptide à balayage assistée
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March 24, 2017
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Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.
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Chen, C., Chang, C. Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope. J. Vis. Exp. (121), e55417, doi:10.3791/55417 (2017).
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