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Acute en chronische modellen van hyperglycemie bij zebravis: een methode om de impact van hyperglycemie op neurogenese te beoordelen en de biodistributie van radiolabelde moleculen
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
DOI:

13:35 min

June 26, 2017

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Capítulos

  • 00:00Título
  • 01:31Establishing a Model of Acute Hyperglycemia in Zebrafish
  • 02:59Establishing a Model of Chronic Hyperglycemia in Zebrafish
  • 03:41Measuring Blood Glucose Levels in Zebrafish
  • 05:00Analyzing Brain Cell Proliferation Following Hyperglycemia
  • 06:24Sample Preparation for Immunohistochemistry
  • 09:09Microscopy
  • 10:05Studying the Impact of Hyperglycemia on Brain Repair Mechanism
  • 11:08Imaging the Bio-distribution of Radiolabeled Molecules by PET-Scan
  • 12:28Representative Results of [18F]-FDG PT/CT Imaging Distribution in Zebrafish
  • 12:48Conclusion

Summary

Tadução automática

Dit werk beschrijft methodes om acute en chronische hyperglycemie modellen in zebravis te bepalen. Het doel is om de invloed van hyperglycemie op fysiologische processen te onderzoeken, zoals constitutieve en schade-geïnduceerde neurogenese. Het werk benadrukt ook het gebruik van zebravis om radioactieve gelabelde moleculen te volgen (hier, [ 18 F] -FDG) met behulp van PET / CT.

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