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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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JoVE Journal Bioquímica
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

DOI:
10.3791/55082-v

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14:53 min

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January 16, 2017

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, , , ,
1Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.

Capítulos

  • 00:00Título
  • 00:56Preparation of Background Electrolyte (BGE) Solution
  • 02:18Sample Preparation
  • 03:45CE System Cleaning
  • 05:46Cartridge Assembly
  • 09:41Preparation of Buffer Trays
  • 10:44CE System Components Assembly
  • 12:10Instrument Methods Set-up
  • 13:21Data Acquisition and Processing
  • 14:11Conclusion

Summary

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Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.

Tags

Keywords: Capillary ElectrophoresisMonoclonal AntibodyIsoformsHPMCBuffer SolutionSample PreparationCE Instrument

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