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Anvendelse af Soft-agar overlay teknik til at screene for bakterielt produceret inhibitoriske forbindelser
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds

Anvendelse af Soft-agar overlay teknik til at screene for bakterielt produceret inhibitoriske forbindelser

30,904 Views

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06:38 min

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January 14, 2017

DOI:

10.3791/55064-v

DOI:
10.3791/55064-v

•

06:38 min
January 14, 2017

•

17 Views
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1School of Plant Sciences,University of Arizona

Tags

Soft-agar OverlayBacterially Produced Inhibitory CompoundsMicrobial EcologyAntagonistic Bacterial InteractionsP. SyringaeKing’s Medium BMitomycin CSodium ChloridePolyethylene Glycol (PEG)Chloroform ExtractionBacterial CultureScreening

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Hockett, K. L., Baltrus, D. A. Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds. J. Vis. Exp. (119), e55064, doi:10.3791/55064 (2017).

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