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Biologia
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Isolierung und Charakterisierung von Mikrovesikeln aus peripherem Blut
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and Characterization of Microvesicles from Peripheral Blood
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Isolierung und Charakterisierung von Mikrovesikeln aus peripherem Blut

22,475 Views

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06:03 min

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January 06, 2017

DOI:

10.3791/55057-v

DOI:
10.3791/55057-v

•

06:03 min
January 06, 2017

•

9 Views
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1Department of Hematology/Medical Oncology,University Medical Center Göttingen

Summary

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Extracellular vesicles present in blood have been suggested as novel biomarkers for various diseases. Here, we present a protocol for the isolation of large plasma membrane-derived microvesicles from peripheral blood samples and their subsequent analysis by conventional flow cytometry and Western Blotting.

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MicrovesiclesPeripheral BloodPlasmaCentrifugationFlow CytometryCharacterizationProtein ConcentrationProinvasiveMetastasisTumor PatientsEDTAPBSFCSAntibody

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Cite this Article

Menck, K., Bleckmann, A., Schulz, M., Ries, L., Binder, C. Isolation and Characterization of Microvesicles from Peripheral Blood. J. Vis. Exp. (119), e55057, doi:10.3791/55057 (2017).

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