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瞬时转染293T细胞复制缺陷型逆转录病毒生产
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Biologia
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JoVE Journal Biologia
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
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瞬时转染293T细胞复制缺陷型逆转录病毒生产

DOI:
10.3791/550-v

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09:40 min

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December 04, 2007

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,
1Molecular Oncology Research Institute,Tufts University

Capítulos

  • 00:08Introduction
  • 00:51Preparation of 293T cells
  • 03:51Preparation of transfection mixture
  • 04:52Transfection
  • 07:02Collection of virus
  • 08:00Discussion

Summary

Tadução automática

Tadução automática

这种技术演示了一个有效的方式来准备复制缺陷型逆转录病毒编码的人类癌基因的股票，随后诱导骨髓增殖性疾病的小鼠模型中使用。

Tags

Replication-defective RetrovirusTransient Transfection293T CellsHuman Myeloproliferative DiseasesOncogenesBcr-AblGrowth Factor Receptor-derived OncogenesZNF198-FGFR1Bcr-PDGFRαMouse ModelBone Marrow CellsRetroviral VectorGag-pol-env Packaging FunctionsCalcium Phosphate TransfectionChloroquineEcotropic VirusCulture SupernatantCell-linesTransformation StudiesIn Vitro StudiesPrimary CellsMouse Model

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