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Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

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06:25 min

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December 23, 2016

DOI:

10.3791/54881-v

DOI:
10.3791/54881-v

•

06:25 min
December 23, 2016

•

3 Views
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1Department of Pathology,University of Miami

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Simian Immunodeficiency VirusCD8+ T-cellsRhesus MacaquesPeptide-MHC-I Tetramer StainingT-cell ImmunologyAntigen-specific CD8 T-cellsFlow CytometryPBMCProtein Kinase InhibitorTetramer Master MixStaining Master MixParaformaldehyde

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Gonzalez-Nieto, L., Domingues, A., Ricciardi, M., Gutman, M. J., Maxwell, H. S., Pedreño-Lopez, N., Bailey, V., Magnani, D. M., Martins, M. A. Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining. J. Vis. Exp. (118), e54881, doi:10.3791/54881 (2016).

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