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Thermostabilization, Expression, rening och kristallisation av den mänskliga Serotonin Transporter Bound till<em> S</em> -citalopram
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
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Thermostabilization, Expression, rening och kristallisation av den mänskliga Serotonin Transporter Bound till<em> S</em> -citalopram

14,588 Views

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12:21 min

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November 27, 2016

DOI:

10.3791/54792-v

DOI:
10.3791/54792-v

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12:21 min
November 27, 2016

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9 Views
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1Vollum Institute,Oregon Health & Science University, 2Graduate Group in Biophysics,University of California, San Francisco, 3Howard Hughes Medical Institute,Oregon Health & Science University

Tags

Serotonin TransporterS-citalopramThermostabilizationExpressionPurificationCrystallizationMembrane ProteinsDrug TargetsNeurotransmitter TransportersReceptorsChannelsSoluble ProteinsHEK293S GnTIDNA TransfectionCitalopramC12MCHSProtease InhibitorsTritiated CitalopramBSAHis-tag AffinitySPA BeadsSertraline

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Coleman, J. A., Green, E. M., Gouaux, E. Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram. J. Vis. Exp. (117), e54792, doi:10.3791/54792 (2016).

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