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一种用于丝状真菌菌落细菌生物膜的定性多尺度分析的新方法利用共聚焦和电子显微镜

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

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一种用于丝状真菌菌落细菌生物膜的定性多尺度分析的新方法利用共聚焦和电子显微镜

DOI:

10.3791/54771-v

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09:45 min

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January 25, 2017

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Cora Miquel Guennoc, Christophe Rose, Frédéric Guinnet, Igor Miquel, Jessy Labbé, Aurélie Deveau

¹Interactions Arbres – Microorganismes, UMR1136,INRA Université de Lorraine, ²Ecologie et Ecophysiologie Forestières – PTEF, UMR 1137,INRA Université de Lorraine, ³Biosciences Division,Oak Ridge National Laboratory

Capítulos

00:05Título
00:50Preparation of Bacterial Fungal Cultures
02:11Preparation of In Vitro Biofilm of Bacteria on the Fungal Colony
03:41Sample Preparation for Confocal Microscopy Analysis of Biofilm Formation
05:35Confocal and Electron Microscopic Analysis
07:07Results: Meso- and Micro-scale Analysis of P. fluorescens BBc6 on L. bicolor
08:29Conclusion

Summary

Tadução automática

这个协议描述成长和定性通过共聚焦和电镜分析关于真菌菌丝细菌生物膜的新方法。

Tags

Keywords: Bacterial Biofilm Filamentous Fungal Colonies Confocal Microscopy Electron Microscopy Multi-scale Analysis Cellophane Membrane EDTA Fungal Pre-culture P. Fluorescens Potassium Phosphate Buffer

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