Journal
/
Imunologia e Infecção
/
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

DOI:
10.3791/54720-v

•

10:10 min

•

November 08, 2016

•

, , ,
1Department of Microbiology and Immunology,The Pennsylvania State University College of Medicine

Capítulos

  • 00:05Título
  • 00:43In Vitro Antigen-specific Inducible Pluripotent Stem Cell (iPSC)-T Regulatory Cell (T-reg) Differentiation
  • 04:00In Vivo iPSC Maturation and Autoimmune Arthritis Suppression and Analysis
  • 06:24Results: Representative Antigen-specific iPSC-T-reg Mediated Arthritis Suppression
  • 08:54Conclusion

Summary

Tadução automática

Tadução automática

We present here a method to develop functional antigen (Ag)-specific regulatory T cells (Tregs) from induced pluripotent stem cells (iPSCs) for immunotherapy of autoimmune arthritis in a murine model.

Tags

Keywords: Stem CellRegulatory T CellsAutoimmune ArthritisImmunotherapyAntigen-specificInduced Pluripotent Stem Cells (iPSCs)OP9 Feeder CellsFlt3-LigandIL-7

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54720?article_type=v

Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
check_url/pt/54720?article_type=v

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
check_url/pt/54720?article_type=v

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
check_url/pt/54720?article_type=v

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells
check_url/pt/54720?article_type=v

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
check_url/pt/54720?article_type=v

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
check_url/pt/54720?article_type=v

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
check_url/pt/54720?article_type=v

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
check_url/pt/54720?article_type=v

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
check_url/pt/54720?article_type=v

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

Read Article