/
/
איתור של<em> Trypanosoma brucei</em> מיתוג גליקופרוטאין Surface Variant ידי מגנטי Activated Cell מיון cytometry זרימה
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
איתור של<em> Trypanosoma brucei</em> מיתוג גליקופרוטאין Surface Variant ידי מגנטי Activated Cell מיון cytometry זרימה
8,604 Views
•
09:45 min
•
October 19, 2016
DOI:
Summary
Automatically generated
African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.
Read Article
Cite this Article
Schulz, D., Mugnier, M. R., Boothroyd, C. E., Papavasiliou, F. N. Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry. J. Vis. Exp. (116), e54715, doi:10.3791/54715 (2016).
Copy