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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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JoVE Journal Genética
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

8,010 Views

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06:53 min

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November 11, 2016

DOI:

10.3791/54673-v

DOI:
10.3791/54673-v

•

06:53 min
November 11, 2016

•

1 Views
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1Department of Medical Specialties, Division of Endocrinology, Diabetes, Hypertension and Nutrition, Diabetes Center,University of Geneva Medical School, Institute of Genetics and Genomics in Geneva (iGE3), 2Population Epidemiology Unit (UEP), Community Medicine,Geneva University Hospital

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Circadian ClockBioluminescenceHormone SecretionPrimary Cell CultureLentiviral TransductionSynchronizationCLOCKInsulin SecretionMyokine Secretion

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Petrenko, V., Saini, C., Perrin, L., Dibner, C. Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures. J. Vis. Exp. (117), e54673, doi:10.3791/54673 (2016).

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