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Subcellulaire Patch-clamp opnamen in het somatodendritische Domein van nigrale Dopamine Neuronen
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Subcellular Patch-clamp Recordings from the Somatodendritic Domain of Nigral Dopamine Neurons
DOI:

09:17 min

November 02, 2016

Capítulos

  • 00:05Título
  • 00:36Preparation of Brain Slices
  • 02:11Dendritic Cell-attached Recording
  • 05:58Post Hoc Visualization of Biocytin-filled Neurons
  • 07:07Results: Cell-attached Recording in a DA Neuron
  • 08:13Conclusion

Summary

Tadução automática

Subcellular patch-clamp recordings offer the possibility to investigate the functional properties of dendrites. However these fine structures are not easily accessible due to their small diameter. The protocol described here aims to facilitate the collection of stable and reliable recordings from the dendrites of dopamine neurons in slices in vitro.

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