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<em> 체외</em> 임피던스 분광학을 사용하여 혈액 - 뇌 장벽 모델 : T 세포 - 내피 세포 상호 작용에 초점
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction
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<em> 체외</em> 임피던스 분광학을 사용하여 혈액 - 뇌 장벽 모델 : T 세포 - 내피 세포 상호 작용에 초점

11,000 Views

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10:17 min

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December 08, 2016

DOI:

10.3791/54592-v

DOI:
10.3791/54592-v

•

10:17 min
December 08, 2016

•

28 Views
, , , , ,
1Department of Neurology,University Hospital Münster, 2Institute of Biochemistry,University of Münster

Summary

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Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.

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Blood-brain BarrierIn Vitro ModelImpedance SpectroscopyT Cell-endothelial Cell InteractionNeuroimmunologyAutoimmune CNS DisordersEAEPrimary Endothelial Cell CultureT Cell ActivationMultiple SclerosisMBMEC

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Kuzmanov, I., Herrmann, A. M., Galla, H., Meuth, S. G., Wiendl, H., Klotz, L. An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction. J. Vis. Exp. (118), e54592, doi:10.3791/54592 (2016).

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