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Spiralganglion Neuron Explantation Kultur und Elektrophysiologie auf Multi-Elektroden-Arrays
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
DOI:

07:51 min

October 19, 2016

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Capítulos

  • 00:05Título
  • 00:54Preparation of MEAs for Culture Experiments
  • 02:11Spiral Ganglion Dissection
  • 03:34Spiral Ganglion Explant Culture on MEAs
  • 04:37Electrophysiological Recordings to Investigate Spontaneous and Electrode Stimulation Dependent Activity
  • 05:52Results: Data Recordings on MEAs
  • 07:01Conclusion

Summary

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We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.

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