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Identificatie van kleine synthetische molecules bindende eiwitten in een Inheemse cellulaire omgeving door live-cell fotoaffiniteitslabeling
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Identificatie van kleine synthetische molecules bindende eiwitten in een Inheemse cellulaire omgeving door live-cell fotoaffiniteitslabeling
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September 20, 2016
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We describe here a method for identification of small molecule-binding proteins using photoaffinity labeling. The advantage of this technique is that binding and covalent labeling of the target proteins occurs within the live cellular environment, removing the risk of disrupting native protein structure and binding conditions upon cell lysis.
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Head, S. A., Liu, J. O. Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling. J. Vis. Exp. (115), e54529, doi:10.3791/54529 (2016).
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