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Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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Bioquímica
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JoVE Journal Bioquímica
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

12,334 Views

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10:49 min

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September 20, 2016

DOI:

10.3791/54529-v

DOI:
10.3791/54529-v

•

10:49 min
September 20, 2016

•

10 Views
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1Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 2Department of Oncology,Johns Hopkins University School of Medicine

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Small MoleculeBinding ProteinsPhotoaffinity LabelingLive-cellMolecular MechanismDrug TargetProtein IdentificationNative Cellular Environment

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Cite this Article

Head, S. A., Liu, J. O. Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling. J. Vis. Exp. (115), e54529, doi:10.3791/54529 (2016).

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