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在小鼠实验为细菌性肺炎的诱导和表型非侵入性和技术上非密集型方法
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
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在小鼠实验为细菌性肺炎的诱导和表型非侵入性和技术上非密集型方法

DOI:
10.3791/54508-v

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07:43 min

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September 28, 2016

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1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences (NIEHS),National Institutes of Health

Capítulos

  • 00:05Título
  • 00:53K. pneumoniae Culture Preparation
  • 02:36Murine Intratracheal (i.t.) K. pneumoniae Aspiration
  • 03:26Bronchoalveolar Lavage Fluid (BALF) Collection and Analysis
  • 04:23Bacterial Lung and Peripheral Tissue Load Determination
  • 05:20Results: Representative Murine Systemic and Immune Cell Responses to K. pneumoniae Oropharynx Delivery
  • 06:42Conclusion

Summary

Tadução automática

Tadução automática

几种方法已在文献中的小鼠模型细菌性肺炎进行了描述。在此，我们描述了用于通过移液到口咽细菌接种物的抽吸（ 即 ，吸入）诱导肺炎的非侵入性的，廉价的，快速的方法。对于肺先天免疫应答的评估下游方法也是详细说明。

Tags

Bacterial PneumoniaMouse ModelNon-invasiveKlebsiella PneumoniaeInnate Immune ResponsePulmonary InfectionInfectious DiseaseImmunologyBiosafety Level 2Serial DilutionsOptical DensityCFUIntranasal Inoculation

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