Journal
/
Genética
/
RNA מחייב רומן בידוד חלבונים לפי שיטת RAPID
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Please note that all translations are automatically generated. Click here for the English version.

RNA מחייב רומן בידוד חלבונים לפי שיטת RAPID

8,803 Views

•

11:19 min

•

September 30, 2016

DOI:

10.3791/54467-v

DOI:
10.3791/54467-v

•

11:19 min
September 30, 2016

•

4 Views
,
1Faculty of Biology,Technion – Israel Institute of Technology, 2Department of Biomolecular Sciences,Weizmann Institute of Science

Summary

Automatically generated

RNA-protein interactions lie at the heart of many cellular processes. Here, we describe an in vivo method to isolate specific RNA and identify novel proteins that are associated with it. This could shed new light on how RNAs are regulated in the cell.

Explore More Videos

RNA-binding ProteinsRaPID MethodologyRNA MetabolismRNA RegulationMRNA-associated ProteinsMS2-tagged MRNAMS2 Binding ProteinStreptavidin Binding ProteinCross-linkingRapid PurificationRNA DegradationRapid Lysis BufferBead-beatingCell Lysate

Read Article

Cite this Article

Samra, N., Arava, Y. Novel RNA-Binding Proteins Isolation by the RaPID Methodology. J. Vis. Exp. (115), e54467, doi:10.3791/54467 (2016).

Download .ris file

Copy

Share Video

.

Copy