Journal
/
Biologia do Desenvolvimento
/
איתור תיוג זיקה של אנדוגני רצפטורים מעוברים דג הזברה
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
Please note that all translations are automatically generated. Click here for the English version.

איתור תיוג זיקה של אנדוגני רצפטורים מעוברים דג הזברה

5,493 Views

•

08:39 min

•

August 31, 2016

DOI:

10.3791/54405-v

DOI:
10.3791/54405-v

•

08:39 min
August 31, 2016

•

2 Views
, ,
1Cell and Developmental Biology, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

Summary

Automatically generated

A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

Explore More Videos

Affinity LabelingReceptor DetectionZebrafish EmbryosBiologia do DesenvolvimentoReceptor ExpressionBiochemical PropertiesAFLIPCell BiologyDeyolkingYolk RemovalLigand BindingProtein ExtractionBradford Assay

Read Article

Cite this Article

Molina-Villa, T., Mendoza, V., López-Casillas, F. Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos. J. Vis. Exp. (114), e54405, doi:10.3791/54405 (2016).

Download .ris file

Copy

Share Video

.

Copy