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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

DOI:
10.3791/54345-v

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08:53 min

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August 31, 2016

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,
1INRS-Institut Armand-Frappier, Université du Québec,Institut National de la Recherche Scientifique

Capítulos

  • 00:05Título
  • 01:12Donor and Competitor Bone Marrow Cell Preparation
  • 02:58Donor Hematopoietic Stem Cell (HSC) Equivalent Identification
  • 04:34Bone Marrow Transplants
  • 05:27Peripheral Blood Analysis
  • 06:39Results: Representative Peripheral Blood Chimerism
  • 07:53Conclusion

Summary

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This protocol provides step-by-step guidelines for setting up competitive mouse bone marrow transplant experiments to study hematopoietic stem/progenitor cell function without prior purification of stem cells by cell sorting.

Tags

Competitive Transplants To Evaluate Hematopoietic Stem Cell Fitness Keywords: – Competitive Transplant Assay – Hematopoietic Stem Cells – Bone Marrow – In Vivo – Stem Cell Self-renewal – Cell Sorting – Mixed Bone Marrow Chimeras – Hematopoiesis – Stem Cell Biology – Immunology

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