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RNA及びDNA抽出の両方のためのホルマリン固定パラフィン包埋組織コアの調製
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
DOI:

08:30 min

August 21, 2016

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Capítulos

  • 00:05Título
  • 00:57Tissue Coring
  • 02:40Deparaffinization of the FFPE Tissue Cores
  • 03:45Homogenization of the Deparaffinized Cores
  • 05:07Digestion with Proteinase K and Nucleotide Separation
  • 06:12Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
  • 07:50Conclusion

Summary

Tadução automática

この修​​正された抽出プロトコルは、組織病理学的組織ブロックへの関心をより正確に対象地域からのRNAとDNA収量を向上させます。

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