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Aanbrengen Fluorescence Resonance Energy Transfer (FRET) naar Effector Translocatie Efficiency Onderzoek door<em> Coxiella burnetii</em> Tijdens siRNA Silencing
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
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Aanbrengen Fluorescence Resonance Energy Transfer (FRET) naar Effector Translocatie Efficiency Onderzoek door<em> Coxiella burnetii</em> Tijdens siRNA Silencing

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10:29 min

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July 06, 2016

DOI:

10.3791/54210-v

DOI:
10.3791/54210-v

•

10:29 min
July 06, 2016

•

1 Views
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1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

Summary

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Onderzoek naar de interactie tussen bacteriële pathogenen en hun gastheren is een belangrijk gebied van biologisch onderzoek. Hier beschrijven we de noodzakelijke technieken om effector translocatie door Coxiella burnetii tijdens siRNA gene silencing behulp blam substraat te meten.

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FRETEffector TranslocationSiRNA SilencingCoxiella BurnetiiCellular MicrobiologyHost-pathogen InteractionsChlamydiaSalmonellaE. ColiBeta-lactamaseACCM2HeLa CellsTransfection96-well Plate

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Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).

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