Journal
/
Imunologia e Infecção
/
表型和慢性淋巴细胞性脉络丛脑膜炎病毒感染小鼠中分离激活调节性T细胞的功能分析
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
Please note that all translations are automatically generated. Click here for the English version.

表型和慢性淋巴细胞性脉络丛脑膜炎病毒感染小鼠中分离激活调节性T细胞的功能分析

9,611 Views

•

07:17 min

•

June 22, 2016

DOI:

10.3791/54138-v

DOI:
10.3791/54138-v

•

07:17 min
June 22, 2016

•

1 Views
, ,
1Department of Biochemistry, College of Life Science & Biotechnology,Yonsei University

Summary

Automatically generated

Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells.

Explore More Videos

Regulatory T CellsChronic Lymphocytic Choriomeningitis VirusPhenotypic AnalysisFunctional AnalysisIn Vitro SuppressionCD4+CD25+ CellsCD8+ Responder T CellsAnti-CD3/CD28 BeadsCytokine Production

Read Article

Cite this Article

Park, H. J., Oh, J. H., Ha, S. Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice. J. Vis. Exp. (112), e54138, doi:10.3791/54138 (2016).

Download .ris file

Copy

Share Video

.

Copy