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FRET成像的三维水凝胶
JoVE Journal
Biologia
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JoVE Journal Biologia
FRET Imaging in Three-dimensional Hydrogels
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FRET成像的三维水凝胶

DOI:
10.3791/54135-v

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09:47 min

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August 01, 2016

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, ,
1Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, 2Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, 3Laerdal AS

Capítulos

  • 00:05Título
  • 00:47Hydrogel Preparation
  • 02:303D FRET Imaging
  • 04:35FRET Ratio Calculation
  • 07:11Results: FRET Imaging of Forskolin-treated Cells in Hydrogels
  • 09:08Conclusion

Summary

Tadução automática

Tadução automática

荧光共振能量转移（FRET）成像是用于实时细胞生物学的研究的强有力的工具。这里提出了在使用常规荧光显微镜生理三维（3D）水凝胶的微环境FRET显像细胞的方法。被描述为比例FRET探针产生在激活范围线性比例的分析。

Tags

Keywords: FRET Imaging3D HydrogelsIntracellular SignalingBiologia do DesenvolvimentoPharmacologyRegenerative MedicineBiomaterialsDrug ScreeningWide Field MicroscopyHydrogel CastingCell SuspensionCrosslinkingUV ExposurePDMS DishPhenol-free MediumMicroscope ImagingFRET Ratio

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