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Capítulos
- 00:05Título
- 00:51Gloeobacter violaceus (GLIC) Expression and Purification
- 05:31GLIC Reconstitution in Asolectin Membranes
- 07:33EPR Measurements
- 08:42Results: Characterization of Pentameric Ligand-Gated Ion Channels
- 10:06Conclusion
This article describes methods for site-directed spin labeling and reconstitution of pentameric ligand-gated channels for Electron Paramagnetic Resonance studies. This protocol can be adapted for any membrane protein. The reconstitution method described here can also be used for patch-clamp measurements of macroscopic and single-channel currents in a defined lipid system.
